Dispensador Digital Programable eVol

Sólo tres jeringas XCHANGE^®  para dosificar líquidos de 0.2 a 1000 μL

Las aplicaciones típicas de un sistema eVol® incluyen:

Preparación de Estándares de Calibración, Adición de Patrones Internos, Dosificación precisa de líquidos no acuosos, Manipulación de Líquidos Viscosos, Tóxicos o Corrosivos, Dilución de muestras, Clean-up y concentración de muestras con MEPS...

eVol^® Kit (2910000).

Incluye jeringa eVol^® (2910005), Alimentador (2910012), Soporte (2910010) y 3 jeringas eVol^® XCHANGE^® de 5, 50 y 500μL (2910020, 2910022, 2910024).

Las jeringas eVol^® XCHANGE^® de 5 y 50μL pueden usarse conjuntamente con las agujas BIN de MEPS para cleanUp o preconcentración de muestras por EFS en jeringa.

El Stand Cargador para 1 eVol^® (2910030) es opcional.

2910000

eVol® Kit. Incluye eVol®, Alimentador Universal, Soporte Estándar y 3 jeringas eVol® XCHANGE® (5, 50 y 500μL)

eVol® NMR Edition allows accurate manipulation of small volumes of NMR samples enabling dilution directly in the analysis tube – even in tubes with the smallest internal diameters. eVol® facilitates in-tube sample mixing and enables recovery of precious samples allowing them to be stored in alternative vessels (such as inexpensive vials) rather than being left in valuable tubes. These tubes can now be emptied using eVol®, for washing and re-use.

eVol®, Propuesta de Valor: Para laboratorios que han de dispensar repetidamente líquidos no
acuosos, eVol® es un instrumento de desplazamiento positivo de control digital que mejora la
eficacia del laboratorio y mejora la fiabilidad de los resultados. A diferencia de otros dispensadores
de laboratorio, eVol® es un dispensador manual único que puede ser fácilmente calibrado para dispensar líquidos de manera precisa y exacta independientemente da la habilidad del usuario.

Aplicación de Micro-SPE (MEPS) ON-LINE y OFF-LINE 

Solid-phase extraction (SPE) has revolutionized sample preparation. Variations on the technique offer enhanced recovery, greater speciation and reduced solvent and sample consumption over other techniques. Micro-Extraction Packed Sorbent (MEPS) is the miniaturization of conventional SPE from milliliter to microliter bed volumes that allows SPE to be used with very small samples. The manipulation of the small volumes is achieved with a precision gas tight syringe. With a typical void volume of 7μL, the MEPS elution is compatible with GC and LC inlets making it ideal for integration into an automated sampling system for on-line SPE.

Preparación de Muestras ON-SITE usando MEPS para Análisis de Agua Residual

MEPS uses a barrel insert and needle (BIN) device to reduce Solid-Phase Extraction (SPE) to a micro-scale suitable for small volume samples and for the on-line adaptation of conventional SPE techniques. Because the SPE cartridge (BIN) is incorporated into the needle assembly of a gas-tight syringe, MEPS is also a simple field-portable  SPE device that may be operated manually without need for sampling pumps or, alternatively, may be incorporated into robotic samplers.

MEPS para Contaminantes y Muestras de Pequeño Volumen

• The carrier gas was helium with a nominal flowrate of 1.3 mL/min in constant flowrate mode and a nominal inlet pressure of 10.8 psi.

Aplicación de MEPS acoplado a GC-TOF MS en el Análisis de Retardantes de Llama Bromados en Agua Residual

In last years, miniaturized analytical techniques had gained attention due to its many special
features over classical approaches. Among many advantages, usage of little or no solvent,
increasing of sensitivity of analysis and user-friendly system, should be pointed out. Micro
extraction in packed sorbent (MEPS) is a new technique for miniaturized solid-phase extraction that
can be connected online to gas chromatography (GC) or liquid chromatography (LC) without other sample pre-treatment.1-4 The sample (10–250 μL) is withdrawn through the syringe by an autosampler. When the sample has passed through the solid support, the analytes are adsorbed to
the solid phase packed in a barrel insert and needle (BIN). The solid phase is washed afterwards by water to remove any interfering material, and the elution of analytes is performed directly into the instrument’s injector by organic solvent (in case of GC), or the LC mobile phase. The large injected volume, typically from 10 to 50 μL, may imply the use of programmed temperature vaporiser (PTV) and large volume injection (LVI). Moreover, a large variety of special packed materials in BIN, such restricted access material (RAM) or molecular imprinted polymers (MIPs), can be used.

The Extraction and Analysis of Urinary Antitussive Metabolites using MEPS™ and ESI-LCMSn

Microextraction Packed Sorbent (MEPS™) is an adaptation of SPE into a miniaturized device with a typical void volume of less than 10 μL. With operating volumes of this scale and its compatibility with autosampler syringes, MEPS™ allows the specificity of the solid-phase process to be harnessed for digital chromatography using discontinuous changes in solvent polarity. The eluant volumes are sufficiently small to be injected directly into a HPLC system and therefore permit the on-line use of solid-phase extraction methodology in real time with the HPLC.

MEPS: A New Technique for the Analyisis of Small Brominated and Chlorinated Aromatic Compounds in Wine

The wine industry has long suffered from TCA (2,4,6-trichloroanisole) and TBA (2,4,6 tribromoanisole) contamination or “cork” taint. These compounds more recently has been found to be present in corks, packaging material, cardboard boxes, wooden products and many other materials. Only 1-2 ng/l of TCA or TBA is required to give wine the “musty” aroma associated with cork taint. It has been proposed that chlorophenols are the precursor for TCA (Fig1). As dioxins are known to be present as contaminants in chlorophenol formulations (1) they might therefore also be present in the wine corks, a hypothesis that needs further invesitigation. In this work, the analysis of TCA and TBA is performed by a new technique for sample preparation called Microextraction by Packed Sorbent (MEPS). This technique in combination with different GCMS techniques is described below and compared with other methods.

The application of MEPS for the on-site preparation of water samples

MEPS uses a barrel insert and needle (BIN) device to reduce Solid-Phase Extraction (SPE) to a micro-scale suitable for small volume samples and for the on-line adaptation of conventional SPE techniques. Because the SPE cartridge (BIN) is incorporated into the needle assembly of a gas-tight syringe, MEPS is also a simple field-portable SPE device that may be operated manually without need for sampling pumps or, alternatively, may be incorporated into robotic samplers.
To demonstrate the application of MEPS we report the qualitative field use of C18-MEPS for the sampling of water bodies in both industrial and urban environments. Water samples were extracted on-site and then the MEPS
syringes were transported back to the laboratory overnight for elution and analysis of the retained components.
C18-MEPS was used to extract samples (100-1000 μL) of water from industrial drains and stormwater pipes to recover semi-volatile residues for GCMS analysis (Fig 1-3). The technique was also applied to sampling droplets on surface extracts of paint for non-destructive surface profiling (Fig 2). Analysis of the unknown samples was qualitative but sufficiently sensitive to detect industrial discharge residues associated with grinding coolants, packaging materials and other contaminants. Naturally occurring leachates from vegetation were also detectable. Droplet extraction of a painted surface was performed in reversed-phase mode (water extract on C18 sorbent) and allowed the surface to be profiled for comparative purposes.

The Integration of Microextraction Packed Sorbent (MEPS) into Multidimensional Strategies

LC-GC approaches to analysis are particularly attractive because they combine the selectivity of solidphase
sorbents in the first dimension with the separating power and peak capacity of a capillary GC column in the second and subsequent dimensions. Widespread use of the technique is limited because of the difficultly in desolvating
the stream from the LC dimension without the solvent vapour passing down the GC column in significant quantity.
An alternative approach to elution chromatography in the first dimension is to harness the specificity of the solid-phase process for digital chromatography using discontinuous changes in solvent polarity (Fig 1). Digital chromatography on a small sorbent bed reduces the volume of mobile phase to discrete plugs that are sufficiently small to be injected directly into a GC with a large volume injector or, alternatively, subsampled into a conventional split/splitless injector.
Microextraction Packed Sorbent (MEPS) is an adaptation of SPE that incorporates all the desirable characteristics
into a miniaturized device with a typical void volume of less than 10 μL. With operating volumes of this scale and its compatibility with autosampler syringes, the MEPS format is the ideal for a digital LC - elution GC approach to analysis.
In this application, we use the selectivity of an argentation sorbent to speciate a mixture of fatty acid methyl
esters on the basis of unsaturation in the first dimension and then to separate groups by conventional non-polar GCMS in the second dimension.

Argentation Based (MEPS) for the Analysis of FAMES by GCMS

Micro Extraction by Packed Sorbent (MEPS) is an adaptation of SPE that incorporates all the desirable characteristics into a miniaturized device with a typical void volume of less than 10 μL. With operating volumes of this scale and its compatibility with autosampler syringes, MEPS allows the specificity of the solid-phase process to be harnessed for digital chromatography using discontinuous changes in solvent polarity (Fig 1). The eluant volumes are sufficiently small to be injected directly into a GC with a large volume injector or, alternatively, subsampled into a conventional split/splitless injector and therefore MEPS can be used as a digital LC - elution GC approach to analysis.
In this application, we use the selectivity of an argentation sorbent to speciate a mixture of fatty acid methyl
esters on the basis of unsaturation in the first dimension and then to separate groups by conventional non-polar GCMS in the second dimension.

Microextraction Packed Sorbent (MEPS): Analysis of Food and Beverages

MEPS is a development of conventional SPE that has miniaturised the sorbent bed so that it can be incorporated into the sample path without voids. Typically, a MEPS method reduces sample and reagent consumption by several orders of magnitude over conventional methods. Extraction performance is comparable to conventional SPE because the MEPS sorbent bed retains the same dimensional ratios of the conventional device and adaption of existing methods is achieved by scaling all steps in proportion to the bed volumes (typically 1 mL for SPE and 10 μL for MEPS). The small scale of the MEPS device allows elution in a small volume and so the entire extract may be analyzed rather than only using a portion of the prepared extract in a conventional experimental design. SPE and MEPS are not the same as SPME or SBSE techniques. The former rely on solvent desorption and use a mono-layer extraction surface with a very large surface area. The latter are immobilised liquid extraction techniques that are typically used in thermal desorption mode.

Micro Extraction by Packed Sorbent (MEPS™) is an adaptation of SPE into a miniaturized device with a typical void volume of less than 10 μL. With operating volumes of this scale and its compatibility with autosampler syringes, MEPS™ allows the specificity of the solid-phase process to be harnessed for digital chromatography using discontinuous changes in solvent polarity. The eluant volumes are sufficiently small to be injected directly into a HPLC system and therefore permit the use of solid-phase extraction methodology in real time with the HPLC.
In this application, we describe the analysis of naturally voided human urine samples that were collected following the administration of single doses of dextromethorphan for both the parent drug and its urinary metabolites. The effectiveness of MEPS™ is compared with the same sample prepared off-line using conventional cartridge SPE. The method was used to demonstrate the effectiveness of MEPS™ for extraction of biological fluids for LCMS analysis.